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Showing 21-30 of 309 paper
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Holm,2010ExperimentsCofactor Perturbations E. coli K-12 MG1655 Affymetrix E. coli Genome 2.0 Array Peter R. Jensen
Cao,2010ExperimentsRNA amplificationE. coli K-12 (DH5-alpha)Escherichia coli 31K spotted oligonucleotide array (CapitalBio Corp. (Beijing, China)) Three target preparation methods, including the direct labeling, polyadenylation-involved oligo-dT priming, and random priming amplification (respectively referred to as DL, PAOD, and RPA hereafter) were evaluated through expression profiling of a heat shock model of Escherichia coli.Liang Zhang
Lambert,2010ExperimentsResponse to Bdellovibrio infectionE. coli S17-1Panorama (Sigma Genosys)126 genes are reported as significantly regulated. However, 121 of these genes have less than 2-fold response and are below the EcoArray cut-off of 2-fold. Two genes below the cut-off, treA and creB, were validated and are still below the cut-off. cstA was also validated, but the RT-PCR results were less than 2-fold. R. E. Sockett
Yu,2010ExperimentsTriclosan resistant E. coliE. coli IFN4E. coli chip (Microbial Genomics & Applications Center, KRIBB, Korea) A microarray analysis of triclosan-resistant E. coli was completed.Jae-Gu Pan
Lee,2010Experimentscis-dichloroethylene; tress-related proteins E. coli K-12 BW25113 Affymetrix E. coli Antisense Genome Array; Affymetrix E. coli Genome 2.0 Array; A whole-transcriptome study was completed to study and characterize how E. coli copes with the stress associated with remediation. Thomas K. Wood
Tehranchi,2010ExperimentsDksA; DNA Replication and Transcription Machinery E. coli K-12 MG1655 Custom Agilent Escherichia coli 4 x 44k Gene Expression Array Jue D. Wang
Kim,2010Experimentstoxin/antitoxin pair; MqsR/MqsA E. coli K-12 BW25113Affymetrix E. coli Antisense Genome Array Three whole-transcriptome studies were completed to identify genes in E. coli K-12 that are regulated by the MqsR/MqsA toxin/antitoxin pair.Thomas K. Wood
Wang,2010ExperimentsCell Physiology; YidC depletionE. coli K-12 (MC1060); JS7131(YidC depleted) vs. JS71 (wild-type) Affymetrix E. coli Genome 2.0 GeneChipsYidC depletion affects membrane protein insertion in E. coli and causes growth defects. Microarray analysis showed that 163 genes were up-regulated and 99 were down-regulated during growth in a yidC depletion strain. Results were verified for a subset of the selected genes using real-time PCR. Ross E. Dalbey
Bhomkar,2010ExperimentsFimbrial adhesionE. coli K-12 CSH150Corning epoxide slidesAuthors used 1.5 fold cut-off with p-value >0.01.Prasanna Bhomkar
Rutherford,2010ExperimentsStress response; n-Butanol; toxicityE. coli K-12[DH1 (ATCC 33849)] Spotted Oligonucleotides; In-HouseThe n-butanol stress response contains components found in other stress responses in E. coli. Several mutants were tested for increased sensitivity to n-butanol and key genes involved in oxidative stress relief were identified. Gene expression was measured at multiple time points: 30 min, 80 min, and 195 min. qPCR validation was also completed at multiple time points for selected genes: 5 min, 30 min, 80 min, 185 min, and 260 min.Aindrila Mukhopadhyay