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Showing 81-90 of 309 paper
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Fabich,2008Experimentscarbon nutrition; ArrayExpress E. coli O157:H7 (strain EDL933);E. coli K-12 MG1655, E. coli ECOR26, and E. coli Nissle Affymetrix GeneChip custom microarrays (E. coli MG1655 and E. coli EDL933) Total RNA from E. coli MG1655 Strr Nalr (nalidixic acid-resistant) or E. coli EDL933 Strr Nalr was extracted from 10 ml of cells grown to an OD600 of 0.4 on MOPS minimal medium containing glucose (0.2% [wt/vol]) or cecal mucus (10 mg ml?1) as the sole carbon source.Tyrrell Conway
Laubacher,2008ExperimentsCell Envelope Stress Response E. coli K-12 MG1655 Affymetrix GeneChip E. coli Genome 2.0 arrayThe data represents a global gene expression analysis of E. coli stress responses activated by inhibition of specific penicillin-binding proteins amdinocillin (mecillinam) and cefsulodin independently and combined. Strains were grown in Luria Bertani (LB) broth at 37°C with agitation.Sarah E. Ades
Kannan,2008ExperimentsRapid acid treatment E. coli K-12 W3110 Affymetrix antisense E. coli arrays The data represent genes that were up-regulated following an acid-shift at 1 min, 5 min, or 10 min.Joan L Slonczewski
Durfee,2008Experimentsstringent responseE. coli K-12 MG1655Affymetrix antisenseAuthor considered genes significantly regulated if log(2) ratio was +/- 1.5 and the control average log(2) signal intensity was greater than 9.Ding Jun Jin
Carter,2008Experimentscomparative genomics hybridisation E. coli K-12 MG1655; E. coli O157:H7 Sakai; E. coli O157:H7 (EDL933) Array-ready Oligo Set v 1.0; Operon Muna F Anjum
Deana,2008ExperimentsRppH mutantE. coli K-12 BW25113Affymetrix, E. coli Genome array 2.0Additionally, the ppa transcript was increased in the absence of active RppH; a 1.9 fold increase was observed with microarray data, which is below the 2-fold cut off, but a 4.8 fold increase for ppa was observed when assayed by Northern analysis.Joel G. Belasco
Zoetendal,2008ExperimentsTannin resistance E. coli BW13711 Affymetrix E. coli Genome 2.0 array A microarray analysis was completed on cells in MOPS medium with 0.4% glucose and 1% WTE, an aqueous extract from Acacia mearnsii (black wattle) bark. Alexandra H. Smith
White-Ziegler,2008ExperimentsLow temperature; RpoS-dependent genes E. coli K-12 MC4100 3DNA Array 350 RP Expression Array Detection kit The data represents gene expression over long-term growth at 23 and 37 degrees Celsius. A total of 297 genes were up-regulated at 23 degrees, while 126 genes were more highly expressed at 37 degrees.Christine A. White-Ziegler
Vijayendran,2008Experimentsmolecular evolution E. coli K-12 MG1655 and DH10B E. coli K12 V2 OciChip™; Ocimum All cells were grown in M9 minimal medium supplemented with 4 g/l glucose or galactose at 37 degrees C.Chandran Vijayendran
Hibi,2007ExperimentsyhbC; xylitol productionE. coli K-12 BW25113IntelliGene E. coli CHIP version 2.0 (TakaraBio)This publication is concerned with improving NADPH-dependent bioconversion. The authors used transcriptome analysis of a yhbC gene knockout (found to produce xylitol) in the BW25113 background containing K.lactis NADPH-dependent xylose reductase gene XYL1 and an additional chromosomal E. coli xylE instead of xylA (the additional xylE is in the chromosomal location of xylA).Hideo Mori