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Showing 81-90 of 309 paper
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Hayes,2006ExperimentsO2 limitation E. coli K-12 strain W3110 Affymetrix E. coli K-12 antisense arrays The study measured the pH dependence of gene expression in oxygen-limited cultures (Oxygen-limited growth as a function of pH). Cells were cultured at 37 degrees C in closed tubes containing potassium-modified Luria broth (LBK) buffered at pH 5.7, pH 7.0, and pH 8.5, and were harvested at OD600=0.2. Joan L Slonczewski
Blanchard,2007Experimentssuperoxide; soxRE. coli K-12 MG1655Affymetrix GeneChip 2.0 Publication uses microarray as initial step to determine biological significance of gene clustering. Data can also be accessed in GEO accession #GSE6992. Jeffrey Blanchard
Fabich,2008Experimentscarbon nutrition; ArrayExpress E. coli O157:H7 (strain EDL933);E. coli K-12 MG1655, E. coli ECOR26, and E. coli Nissle Affymetrix GeneChip custom microarrays (E. coli MG1655 and E. coli EDL933) Total RNA from E. coli MG1655 Strr Nalr (nalidixic acid-resistant) or E. coli EDL933 Strr Nalr was extracted from 10 ml of cells grown to an OD600 of 0.4 on MOPS minimal medium containing glucose (0.2% [wt/vol]) or cecal mucus (10 mg ml?1) as the sole carbon source.Tyrrell Conway
Conrad,2010ExperimentsRegulatory adaptation E. coli K-12 MG1655 Affymetrix GeneChip E. coli 2.0 A transcriptome analysis of small deletions within the rpoC gene encoding the B-subunit of RNA polymerase. MG1655 was subjected to adaptive evolution for 25 days in glycerol M9 minimal medium (GMM) at 30 degrees C to OD600=0.3. Robert Landick
Laubacher,2008ExperimentsCell Envelope Stress Response E. coli K-12 MG1655 Affymetrix GeneChip E. coli Genome 2.0 arrayThe data represents a global gene expression analysis of E. coli stress responses activated by inhibition of specific penicillin-binding proteins amdinocillin (mecillinam) and cefsulodin independently and combined. Strains were grown in Luria Bertani (LB) broth at 37°C with agitation.Sarah E. Ades
Nobre,2009ExperimentsCORM; antimicrobial action of a carbon monoxide E. coli K-12 MG1655 Affymetrix GeneChip E. coli Genome 2.0 Arrays The data represents an analysis of the gene expression changes occurring when E. coli, grown both aerobically and anaerobically, is treated with the carbon monoxide-releasing molecule CORM-2. Lígia M. Saraiva
Portnoy,2010ExperimentsAerobic-Anaerobic Shift E. coli K-12 MG1655 vs. ECOM4LA Affymetrix GeneChip E. coli Genome 2.0 arrays The data represents a genome-wide transcriptome studies of wild-type and ECOM4LA (Escherichia coli cytochrome oxidase mutant 4 (del:cydAB, cyoABCD, cbdAB, and ygiN)) cells under aerobic and anaerobic conditions. Bacterial strains were cultured at 37 degrees C in M9 medium (4 g/L glucose) to OD600=0.6 for the WT and 0.25 for ECOM4LA.Bernhard �. Palsson
King,2010Experimentsacid tolerance responsesE. coli K-12 MG1655; E. coli O157:H7 SakaiAffymetrix GeneChip E. coli Genome 2.0 arrays Thea King
Bianco,2006ExperimentsIndole-3-acetic acid (IAA)E. coli K-12 MG1655Affymetrix GeneChip E. coli genome arraysR. Defez
Zhao,2010Experimentsflagellar biosynthesis E. coli K-12 MG1655 Affymetrix GeneChipR E. coli Antisense Genome Arrays The DNA microarray results showed that a change of the intracellular level of a given sigma factor will cause a change of the transcriptional level of genes dependent on the sigma factor.Richard R. Burgess