GenePage for the bglF gene of Escherichia coli K-12

Primary Gene Name: bglF
EcoGene Accession Number: EG10115
K-12 Gene Accession Number: ECK3715
MG1655 Gene Identifier: b3722
Gene Name Mnemonic: beta-Glucoside
Alternate Gene Symbols: bglC; bglS
Description: PTS system EIIABC enzyme, beta-glucoside phosphotransferase; BglG kinase/dephosphorylase; membrane-bound protein; binds BglG
  # bp Upstream # bp Downstream
MW: 66482.97 ---------625 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3903720
Left Intergenic Region

Name: bglB_bglF

Length: 18 bp gap

Orientation: Codirectional-

Left_end: 3903702

Right_end: 3903719

Centisome: 84.10

Genomic Address
Counterclockwise
Minute or Centisome (%) = 84.10
Right End: 3905597
Right Intergenic Region

Name: bglF_bglG

Length: 133 bp gap

Orientation: Codirectional-

Left_end: 3905598

Right_end: 3905730

Centisome: 84.14

BglF is probably phosphorylated at His547 in the C-terminal EIIA domain by phospho-HPr (PtsH); His547 then transfers the phosphate to Cys24 in the N-terminal EIIB domain which then transfers the phosphate to BglG, inactivating it in the absence of the beta glucoside sugar, or to the sugar during transport. bglF is part of the cryptic bglGFBH operon. The bgl operon promoter is silenced by H-NS, represssed by Fis and activated by CRP-cAMP.

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BamHI EcoRI HindIII