GenePage for the bioC gene of Escherichia coli K-12

Primary Gene Name: bioC
EcoGene Accession Number: EG10119
K-12 Gene Accession Number: ECK0766
MG1655 Gene Identifier: b0777
Gene Name Mnemonic: Biotin
Alternate Gene Symbols: None
Description: Malonyl-ACP O-methyltransferase, SAM-dependent; biotin synthesis blocked prior to pimeloyl CoA formation
  # bp Upstream # bp Downstream
MW: 28276.20 ---------251 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 811522
Left Intergenic Region

Name: bioF_bioC

Length: 14 bp overlap

Orientation: Codirectional+

Left_end: 811522

Right_end: 811535

Centisome: 17.48

Genomic Address
Clockwise
Minute or Centisome (%) = 17.48
Right End: 812277
Right Intergenic Region

Name: bioC_bioD

Length: 8 bp overlap

Orientation: Codirectional+

Left_end: 812270

Right_end: 812277

Centisome: 17.50

A previous suggestion that BioC may methylate malonyl-ACP instead of malonyl-CoA was confirmed using the BioC enzyme from B. cereus and E. coli malonyl-ACP in vitro because active native BioC cannot be purified; the B. cereus enyzme complements an E. coli bioC mutation thus validating the function of the E. coli BioC enzyme in vivo (Lin, 2012). BioC and BioH are required to synthesize pimeloyl-ACP, a biotin precursor, utilizing the enzymes of fatty acid synthesis to add acyl groups to malonyl-CoA in two cycles to form pimeloyl-ACP methyl ester; BioC may methylate malonyl-ACP instead of malonyl-CoA (Lin, 2010).

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII