GenePage for the chbA gene of Escherichia coli K-12

Primary Gene Name: chbA
EcoGene Accession Number: EG10142
K-12 Gene Accession Number: ECK1734
MG1655 Gene Identifier: b1736
Gene Name Mnemonic: Chitobiose
Alternate Gene Symbols: celC
Description: PTS Enzyme IIA(Chb), N,N'-diacetylchitobiose utilization; trimeric
  # bp Upstream # bp Downstream
MW: 12747.76 ---------116 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1819455
Left Intergenic Region

Name: chbR_chbA

Length: 7 bp gap

Orientation: Codirectional-

Left_end: 1819448

Right_end: 1819454

Centisome: 39.20

Genomic Address
Counterclockwise
Minute or Centisome (%) = 39.20
Right End: 1819805
Right Intergenic Region

Name: chbA_chbC

Length: 50 bp gap

Orientation: Codirectional-

Left_end: 1819806

Right_end: 1819855

Centisome: 39.21

The chb operon is induced by N,N'-diacetylchitobiose. The chb operon was previously thought to encode a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent, but a fully induced operon did not support growth on cellobiose, implying a mutation altering the substrate specificity is needed; several candidate mutations were noted in Genbank, but not proven to alter the specificity of the system (Keyhani, 1997). In contrast, a later study claim that mutations making the operon expression high-level constitutive by chbR and nagC mutations are sufficient for the Cel+ phenotype and that no mutations altering substrate specificity are required (Kachroo, 2007). The overexpressed, but native sequence, ChbA N-terminus was protein sequenced and the methionine is not removed, but the native RBS was not used in a pET vector so ChbA is not in the Verified Set (Keyhani, 2000).

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BamHI EcoRI HindIII