GenePage for the chbF gene of Escherichia coli K-12

Primary Gene Name: chbF
EcoGene Accession Number: EG10144
K-12 Gene Accession Number: ECK1732
MG1655 Gene Identifier: b1734
Gene Name Mnemonic: Chitobiose
Alternate Gene Symbols: celF; ydjD
Description: N,N'-diacetylchitobiose utilization, phospho-chitobiase; can hydrolyze a variety of phospho-beta-glucosides
  # bp Upstream # bp Downstream
Verified Start MW: 50512.50 ---------450 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1817148
Left Intergenic Region

Name: chbG_chbF

Length: 12 bp gap

Orientation: Codirectional-

Left_end: 1817136

Right_end: 1817147

Centisome: 39.15

Genomic Address
Counterclockwise
Minute or Centisome (%) = 39.15
Right End: 1818500
Right Intergenic Region

Name: chbF_chbR

Length: 104 bp gap

Orientation: Codirectional-

Left_end: 1818501

Right_end: 1818604

Centisome: 39.18

The chb operon is induced by N,N'-diacetylchitobiose. The chb operon was previously thought to encode a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent, but a fully induced operon did not support growth on cellobiose, implying a mutation altering the substrate specificity is needed; several candidate mutations were noted in Genbank, but not proven to alter the specificity of the system (Keyhani, 1997). In contrast, a later study claim that mutations making the operon expression high-level constitutive by chbR and nagC mutations are sufficient for the Cel+ phenotype and that no mutations altering substrate specificity are required (Kachroo, 2007).

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BamHI EcoRI HindIII