GenePage for the fhuD gene of Escherichia coli K-12

Primary Gene Name: fhuD
EcoGene Accession Number: EG10305
K-12 Gene Accession Number: ECK0151
MG1655 Gene Identifier: b0152
Gene Name Mnemonic: Ferric hydroxamate uptake
Alternate Gene Symbols: None
Description: Iron(3+)-hydroxamate import ABC transporter periplasmic binding protein; ferrichrome uptake; fhuD mutants are albomycin resistant
  # bp Upstream # bp Downstream
MW: 32998.33 ---------296 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 170575
Left Intergenic Region

Name: fhuC_fhuD

Length: 1 bp overlap

Orientation: Codirectional+

Left_end: 170575

Right_end: 170575

Centisome: 3.67

Genomic Address
Clockwise
Minute or Centisome (%) = 3.67
Right End: 171465
Right Intergenic Region

Name: fhuD_fhuB

Length: 4 bp overlap

Orientation: Codirectional+

Left_end: 171462

Right_end: 171465

Centisome: 3.69

Ferric hydroxamate siderophores all require FhuD for import, e.g. ferrichrome, gallichrome, desferrioxamine, coprogen, aerobactin, and the antibiotic albomycin. Verified Tat substrate: FhuD has a predicted Tat-only (Class I) 30 aa signal peptide (Tullman-Ercek, 2007). The N-terminal sequence in Rohrback (1995), cited in UniProtKB, does not verify this putative SPI cleavage; it is the product of an enterokinase cleavage of a cloning construct. Rohrback (1995) demonstrated proper folding of FhuD in the cytoplasm prior to export, consistent with FhuD needing the Tat pathway, which exports folded proteins.

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BamHI EcoRI HindIII