GenePage for the fucK gene of Escherichia coli K-12

Primary Gene Name: fucK
EcoGene Accession Number: EG10350
K-12 Gene Accession Number: ECK2798
MG1655 Gene Identifier: b2803
Gene Name Mnemonic: Fucose
Alternate Gene Symbols: None
Description: L-fuculose kinase
  # bp Upstream # bp Downstream
MW: 52258.69 ---------472 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2937468
Left Intergenic Region

Name: fucI_fucK

Length: 108 bp gap

Orientation: Codirectional+

Left_end: 2937360

Right_end: 2937467

Centisome: 63.28

Genomic Address
Clockwise
Minute or Centisome (%) = 63.28
Right End: 2938886
Right Intergenic Region

Name: fucK_fucU

Length: 1 bp gap

Orientation: Codirectional+

Left_end: 2938887

Right_end: 2938887

Centisome: 63.32

Although the N-terminal sequence of FucK was reported to be MLXG, consistent with the annotated start MLSGYIAGAIMK (Tonella, 1998), FucK has been removed from the Verified Set and its start site has been reset to M11-K12, removing the first 10 amino acids, based on three strong lines of bioinformatics evidence: (1) all protein sequence alignments to FucK orthologs show that the MK- start is conserved but not the upstream MLSG- start, (2) the conserved start has a very likely RBS (AGGAGCGATT-ATG-AAA) and (3) it removes a 12 base overlap with REP204, which was a unique and unlikely scenario. The source of this likely error is unknown but the 2D gel spot identified as FucK may need further verification, i.e. more unique N-terminal sequence data, to resolve the discrepancy.

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BamHI EcoRI HindIII