GenePage for the gltA gene of Escherichia coli K-12

Primary Gene Name: gltA
EcoGene Accession Number: EG10402
K-12 Gene Accession Number: ECK0709
MG1655 Gene Identifier: b0720
Gene Name Mnemonic: Glutamate
Alternate Gene Symbols: gluT; icdB
Description: Citrate synthase; hexameric
  # bp Upstream # bp Downstream
Verified Start MW: 48014.99 ---------427 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 753185
Left Intergenic Region

Name: ybgD_gltA

Length: 389 bp gap

Orientation: Codirectional-

Left_end: 752796

Right_end: 753184

Centisome: 16.22

Genomic Address
Counterclockwise
Minute or Centisome (%) = 16.23
Right End: 754468
Right Intergenic Region

Name: gltA_sdhC

Length: 708 bp gap

Orientation: Divergent

Left_end: 754469

Right_end: 755176

Centisome: 16.25

Five active site mutations were tested and found to lower GltA in vitro turnover rates including R388L, affecting the orientataion of the oxaloacetate substrate binding; R407L, which allows GltA to metaboliize the competitive inhibitor alpha-ketoglutarate; H265A and D363A, affecting steady-state kinetics; and F384A, affecting the allosteric binding of acetyl-CoA (Pereira, 1994).

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BamHI EcoRI HindIII