GenePage for the ilvG' pseudogene of Escherichia coli K-12

Primary Gene Name: ilvG'
EcoGene Accession Number: EG10498
K-12 Gene Accession Number: ECK3760
MG1655 Gene Identifier: b4488, b3768, b3767
Gene Name Mnemonic: Isoleucine-valine (requirement)
Alternate Gene Symbols: ilvO
Description: Pseudogene reconstruction, acetolactate synthase 2 large subunit; valine-insensitive; acetohydroxy acid synthase II; heterotetrameric AHAS-II
  # bp Upstream # bp Downstream
MW: 59284.85 ---------548 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3950560
Left Intergenic Region

Name: ilvX_ilvG

Length: 2 bp gap

Orientation: Codirectional+

Left_end: 3950558

Right_end: 3950559

Centisome: 85.11

Genomic Address join

The following join statement uses GenBank syntax to describe how the reconstructed frameshifted ilvG pseudogene protein sequence was translated from the DNA:

join(3950560..3951543,3951542..3952204)


Clockwise
Minute or Centisome (%) = 85.11
Right End: 3952204
Right Intergenic Region

Name: ilvG_ilvM

Length: 4 bp overlap

Orientation: Codirectional+

Left_end: 3952201

Right_end: 3952204

Centisome: 85.15

A frameshift at codon 328, the defective ilvG allele present in MG1655 and other K-12 strains, has been hypothetically reconstructed and was probably acquired early during laboratory cultivation; the gene designation ilvO indicates a regulatory locus but ilvO mutations are actually alleles of the ilvG gene that correct or compensate for the original ilvG frameshift mutation (Favre, 1976; Baez, 1979; Smith, 1979; Subrahmanyam, 1980a; Berg, 1980; Lawther, 1981; Lawther, 1981; Gray, 1981; Lawther, 1982; Wek, 1987; Lopes, 1989; Burns, 1995; Parekh, 1997). The corresponding Salmonella IlvG N-terminus was Verified by protein sequencing, supporting this start site for E. coli (Schloss, 1985). The N-terminus of overexpressed E. coli IlvG was also sequenced, but the native RBS was not used. In E. coli strains with a functional ilvG, no ptsN phenotypes are seen, indicating that perhaps ptsN is not a real nitrogen source regulator (Reaves, 2011).

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BamHI EcoRI HindIII