GenePage for the groL gene of Escherichia coli K-12

Primary Gene Name: groL
EcoGene Accession Number: EG10599
K-12 Gene Accession Number: ECK4137
MG1655 Gene Identifier: b4143
Gene Name Mnemonic: Growth of phage
Alternate Gene Symbols: groEL; mopA
Description: Chaperonin Cpn60; phage morphogenesis; GroESL large subunit GroEL, weak ATPase; binds Ap4A
  # bp Upstream # bp Downstream
Verified Start MW: 57328.85 ---------548 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4371025
Left Intergenic Region

Name: groS_groL

Length: 43 bp gap

Orientation: Codirectional+

Left_end: 4370982

Right_end: 4371024

Centisome: 94.17

Genomic Address
Clockwise
Minute or Centisome (%) = 94.17
Right End: 4372671
Right Intergenic Region

Name: groL_yjeI

Length: 137 bp gap

Orientation: Codirectional+

Left_end: 4372672

Right_end: 4372808

Centisome: 94.21

groL is an essential gene (Fayet, 1989). The groL mutants in the Keio collection have been shown to harbor an intact copy of groL in addition to the deletion allele (Yamamoto, 2009). Cells deficient in GroEL and GroES lyse due to inability to produce cell wall as a result of the reduced level of DapA, the first enzyme in the DAP synthesis pathway (McLennan, 1998). Growth below 8C is impaired due to GroESL inactivation and can be rescued by transgenic Oleispira antarctica Cpn10/60 (Ferrer, 2003). Overproduction of GroESL can suppress tig/dnaK synthetic lethality at 30 degrees C. GroL lysines K34, K51, K117, K277, K321 and K390 can be modified to N6-succinyllysine (Zhang, 2011). GroL lysines K42, K117, K122, and K425 can be modified to N6-acetyllysine (Yu, 2008; Zhang, 2009). The groSL promoter region binds FNR and groSL expression is repressed by FNR (Federowicz, 2014).

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BamHI EcoRI HindIII