GenePage for the bamE gene of Escherichia coli K-12

Primary Gene Name: bamE
EcoGene Accession Number: EG10952
K-12 Gene Accession Number: ECK2613
MG1655 Gene Identifier: b2617
Gene Name Mnemonic: Beta-barrel assembly machine
Alternate Gene Symbols: smpA; smqA
Description: Lipoprotein stabilizer of BamABCDE OM biogenesis complex
  # bp Upstream # bp Downstream
MW: 12302.03 ---------113 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2753605
Left Intergenic Region

Name: recN_bamE

Length: 148 bp gap

Orientation: Codirectional+

Left_end: 2753457

Right_end: 2753604

Centisome: 59.32

Genomic Address
Clockwise
Minute or Centisome (%) = 59.32
Right End: 2753946
Right Intergenic Region

Name: bamE_ratB

Length: 61 bp gap

Orientation: Convergent

Left_end: 2753947

Right_end: 2754007

Centisome: 59.33

bamE(smpA) mutants are sensitive to rifampin, vancomycin, cholate, and SDS/EDTA, indicating an OM defect (Sklar, 2007). A bamE(smpA) bamB double mutant is non-viable (Sklar, 2007). SmpA is homologous to P. aeruginosa lipoprotein OmlA. The first 19 aa of BamE are predicted to be a type II signal peptide. A mutation of Cys20 severely reduces bamE complementation of vancomycin resistance (Knowles, 2011). Although a direct biochemical demonstration that E. coli BamE is a lipoprotein appears to be lacking, the supporting evidence is very strong.

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BamHI EcoRI HindIII