GenePage for the frsA gene of Escherichia coli K-12

Primary Gene Name: frsA
EcoGene Accession Number: EG11091
K-12 Gene Accession Number: ECK0240
MG1655 Gene Identifier: b0239
Gene Name Mnemonic: Fermentation-respiration switch
Alternate Gene Symbols: yafA
Description: Fermentation-respiration switch protein; PTS Enzyme IIAGlc-binding protein, binds unphosphorylated form; has pNP-butyrate esterase activity in vitro
  # bp Upstream # bp Downstream
MW: 47008.77 ---------414 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 256527
Left Intergenic Region

Name: gpt_frsA

Length: 91 bp gap

Orientation: Codirectional+

Left_end: 256436

Right_end: 256526

Centisome: 5.52

Genomic Address
Clockwise
Minute or Centisome (%) = 5.53
Right End: 257771
Right Intergenic Region

Name: frsA_crl

Length: 57 bp gap

Orientation: Codirectional+

Left_end: 257772

Right_end: 257828

Centisome: 5.55

An frsA mutant has increased cellular respiration on glucose; FrsA overexpression has increased fermentation rate on glucose. The FrsA N-terminus has been verified by MS, fMet is clipped (Koo, 2004). 8/228 FrsA(YafA) peptide masses from MS analysis confirm this start codon and indicate that the initial Met is removed (Maillet, 2007). FrsA peptide masses from MS/MS analysis indicate this is the correct start site and that the initial Met is removed (Guo, 2014).

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BamHI EcoRI HindIII