GenePage for the crfC gene of Escherichia coli K-12

Primary Gene Name: crfC
EcoGene Accession Number: EG11210
K-12 Gene Accession Number: ECK4102
MG1655 Gene Identifier: b4109
Gene Name Mnemonic: Colocalization of the Replication Fork DNA by the Clamp
Alternate Gene Symbols: yjdA
Description: Clamp-binding sister replication fork colocalization protein; dynamin-related protein, homomultimeric; mutational suppressor of yhjH motility defect
  # bp Upstream # bp Downstream
MW: 84371.05 ---------742 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4327135
Left Intergenic Region

Name: yjdM_crfC

Length: 400 bp gap

Orientation: Divergent

Left_end: 4326735

Right_end: 4327134

Centisome: 93.22

Genomic Address
Clockwise
Minute or Centisome (%) = 93.22
Right End: 4329363
Right Intergenic Region

Name: crfC_yjcZ

Length: 4 bp overlap

Orientation: Codirectional+

Left_end: 4329360

Right_end: 4329363

Centisome: 93.27

CrfC(YjdA) binds to the DNA replisome sliding clamp (DnaN) and colocalizes nascent sister replication forks; purified CrfC(YjdA) has very weak GTPase activity; the membrane binding domain of other dynamin proteins is replaced by a DnaN-binding domain including a binding motif at residues Q41-P45; a Q41A mutant loses clamp binding; GTP does not affect clamp binding in vitro; CrfC(YjdA) binds free or bound DnaN but does not bind naked DNA (Ozaki, 2013). The motility defect of yhjH mutations is suppressed by multicopy yfgF and by mutations in ycgR, fliZ, yfiR, yegE, crfC(yjdA), and yjcZ (Girgis, 2007). crfC(yjdA)-yjcZ are predicted to constitute an operon. CrfC(YjdA) has a Pfam PF00350 Dynamin_N domain and is a member of the dynamin GTPase superfamily.

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BamHI EcoRI HindIII