GenePage for the insZ' pseudogene of Escherichia coli K-12

Primary Gene Name: insZ'
EcoGene Accession Number: EG11611
K-12 Gene Accession Number: ECK1234
MG1655 Gene Identifier: b4573, b1240, b1239
Gene Name Mnemonic: INsertion Sequence
Alternate Gene Symbols: ychG; ychR
Description: Pseudogene reconstruction, IS4 transposase family, in ISZ'
  # bp Upstream # bp Downstream
MW: 33808.04 ---------299 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1294426
Left Intergenic Region

Name: tdk_insZ

Length: 281 bp gap

Orientation: Convergent

Left_end: 1294145

Right_end: 1294425

Centisome: 27.88

Genomic Address join

The following join statement uses GenBank syntax to describe how the reconstructed frameshifted insZ pseudogene protein sequence was translated from the DNA:

complement(join(1294426..1294992,1294992..1295141,1295140..1295322))


Counterclockwise
Minute or Centisome (%) = 27.89
Right End: 1295322
Right Intergenic Region

Name: insZ_adhE

Length: 123 bp gap

Orientation: Codirectional-

Left_end: 1295323

Right_end: 1295445

Centisome: 27.91

Two frameshifts (at codons 62 and 111) and an internal deletion of about 150 codons have mutated this homolog of IS4 transposase InsG (442 aa). An internal 152 aa are present in InsG that are apparently deleted in InsZ' (they would be between positions 238 and 239 in the 299 aa hypothetically reconstructed discontinuous InsG' pseudogene protein sequence). This internal deletion also corresponds to residues 243-383 of the 445 aa IS4 transposase homolog VV1_2235 from Vibrio vulnificus (UniProtKB: Q8DAG), which is the closest homolog to InsZ'. InsG is the closest E. coli homolog. There are numerous pseudogene alleles of insZ' in other strains of E. coli, but no wild type insZ alleles have yet been found.

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BamHI EcoRI HindIII