GenePage for the amiA gene of Escherichia coli K-12

Primary Gene Name: amiA
EcoGene Accession Number: EG11823
K-12 Gene Accession Number: ECK2430
MG1655 Gene Identifier: b2435
Gene Name Mnemonic: Amidase
Alternate Gene Symbols: yfeE
Description: N-acetylmuramyl-L-alanine amidase, periplasmic; role in septal cleavage during cell division; activated by EnvC
  # bp Upstream # bp Downstream
MW: 31412.13 ---------289 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2552352
Left Intergenic Region

Name: ypeA_amiA

Length: 213 bp gap

Orientation: Divergent

Left_end: 2552139

Right_end: 2552351

Centisome: 54.98

Genomic Address
Clockwise
Minute or Centisome (%) = 54.99
Right End: 2553221
Right Intergenic Region

Name: amiA_hemF

Length: 3 bp gap

Orientation: Codirectional+

Left_end: 2553222

Right_end: 2553224

Centisome: 55.01

EnvC activates AmiA- and AmiB-mediated in vitro murein cleavage; cell lysis induced by EnvC overproduction requires expression of amiA or amiB (Uehara, 2010). AmiA is a verified Tat substrate (Bernhardt, 2003). AmiA has a predicted Tat-only (Class I) 34 aa signal peptide (Tullman-Ercek, 2007). In amiABC triple mutants, thickened dark bands termed septal peptidoglycan (SP) rings appear at division sites, representing a cell division structure that accumulates when septal synthesis and hydrolysis by the AmiABC amidases are uncoupled (Priyadarshini, 2007). A mutant lacking five murein hydrolases (amiABCmltCD=MHD90) has a delayed lysis phenotype after beta-lactam treatment (Heidrich, 2002).

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BamHI EcoRI HindIII