GenePage for the grxD gene of Escherichia coli K-12

Primary Gene Name: grxD
EcoGene Accession Number: EG12181
K-12 Gene Accession Number: ECK1650
MG1655 Gene Identifier: b1654
Gene Name Mnemonic: Glutaredoxin
Alternate Gene Symbols: ydhD
Description: Monothiol glutaredoxin Grx4 with CGFS motif; substrate for thioredoxin reductase; mutants are hypersensitive to iron depletion
  # bp Upstream # bp Downstream
MW: 12878.76 ---------115 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1733754
Left Intergenic Region

Name: lhr_grxD

Length: 50 bp gap

Orientation: Convergent

Left_end: 1733704

Right_end: 1733753

Centisome: 37.35

Genomic Address
Counterclockwise
Minute or Centisome (%) = 37.35
Right End: 1734101
Right Intergenic Region

Name: grxD_mepH

Length: 333 bp gap

Orientation: Divergent

Left_end: 1734102

Right_end: 1734434

Centisome: 37.36

grxD mutations are synthetic lethal with mutations in the isc operon, suggesting that Grx4 is involved in an alternative FeS cluster assembly pathway; a similar synthetic lethality pattern was observed for the bolA-like gene ibaG(yrbA), but not for bolA (Butland, 2005; Yeung, 2011). Homodimeric monothiol glutaredoxin Grx4 binds FeS and can efficiently transfer FeS clusters to Fdx in vitro (Yeung, 2011). BolA can form a heterodimer in vitro with FeS-bound monothiol glutaredoxin Grx4 but this complex is much less efficient than the FeS-bound Grx4 homodimer in FeS cluster transfer to Fdx (Yeung, 2011). Grx4 is a highly abundant protein with ppGpp-dependent induction during stationary phase or iron depletion (Fernandes, 2005). Not a substrate for glutathione reductase. Although a deletion for grxD is in the Keio collection, it was also reported that grxD is an essential gene that could not be inactivated (Gerdes, 2003; Fernandes, 2005; Baba, 2006). grxD, but not bolA or ibaG(yrbA) mutants are hypersensitive to iron depletion (Yeung, 2011).

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BamHI EcoRI HindIII