GenePage for the bcsA gene of Escherichia coli K-12

Primary Gene Name: bcsA
EcoGene Accession Number: EG12260
K-12 Gene Accession Number: ECK3518
MG1655 Gene Identifier: b3533
Gene Name Mnemonic: Bacterial cellulose synthesis
Alternate Gene Symbols: yhjO; yhjP
Description: Celullose synthase, catalytic subunit; inner membrane protein
  # bp Upstream # bp Downstream
MW: 99784.58 ---------872 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3692618
Left Intergenic Region

Name: bcsB_bcsA

Length: 10 bp gap

Orientation: Codirectional-

Left_end: 3692608

Right_end: 3692617

Centisome: 79.55

Genomic Address
Counterclockwise
Minute or Centisome (%) = 79.55
Right End: 3695236
Right Intergenic Region

Name: bcsA_bcsQ

Length: 4 bp overlap

Orientation: Codirectional-

Left_end: 3695233

Right_end: 3695236

Centisome: 79.61

Inverted membrane vesicles prepared from E. coli cells overexpressing E. coli K-12 BcsA and BcsB can synthesize cellulose in the presence of c-di-GMP (Omadjela, 2013). The bcsQ' domestication in-frame stop codon pseudogene defect in K-12 strain W3110 has been repaired, restoring cellulose production, colony morphology, biofilm architecture, and the divergent bcs operons' functions to a presumably wildtype status (Serra, 2013). BcsA has a C-terminal PilZ domain; the PilZ domain of YcgR has been implicated in cyclic-di-GMP binding. The presence of an in-frame stop codon mutation in the bcsQ' gene abolishes cellulose production and bcs divergent operon function in E. coli K-12.

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BamHI EcoRI HindIII