GenePage for the ghrB gene of Escherichia coli K-12

Primary Gene Name: ghrB
EcoGene Accession Number: EG12272
K-12 Gene Accession Number: ECK3540
MG1655 Gene Identifier: b3553
Gene Name Mnemonic: Glyoxylate/Hydroxypyruvate Reductase
Alternate Gene Symbols: tkrA; yiaE
Description: Glyoxylate/hydroxypyruvate reductase B; 2-ketogluconate reductase; activity higher on hydroxypyruvate than glyoxylate
  # bp Upstream # bp Downstream
Verified Start MW: 35395.50 ---------324 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3717310
Left Intergenic Region

Name: yiaD_ghrB

Length: 103 bp gap

Orientation: Codirectional+

Left_end: 3717207

Right_end: 3717309

Centisome: 80.08

Genomic Address
Clockwise
Minute or Centisome (%) = 80.09
Right End: 3718284
Right Intergenic Region

Name: ghrB_yiaF

Length: 49 bp gap

Orientation: Convergent

Left_end: 3718285

Right_end: 3718333

Centisome: 80.11

Although two groups have verified this start site by protein sequencing, 6/171 peptide masses from MS analysis indicate that an start four codons upstream is utilized; on the other hand, 1/171 peptides did confirm the current start site (Maillet, 2007).

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BamHI EcoRI HindIII