GenePage for the can gene of Escherichia coli K-12

Primary Gene Name: can
EcoGene Accession Number: EG12319
K-12 Gene Accession Number: ECK0125
MG1655 Gene Identifier: b0126
Gene Name Mnemonic: Carbonic ANhydrase
Alternate Gene Symbols: yadF
Description: Carbonic anhydrase, beta class
  # bp Upstream # bp Downstream
MW: 25096.77 ---------220 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 142008
Left Intergenic Region

Name: hpt_can

Length: 40 bp gap

Orientation: Convergent

Left_end: 141968

Right_end: 142007

Centisome: 3.06

Genomic Address
Counterclockwise
Minute or Centisome (%) = 3.06
Right End: 142670
Right Intergenic Region

Name: can_yadG

Length: 108 bp gap

Orientation: Divergent

Left_end: 142671

Right_end: 142778

Centisome: 3.07

can forms a synthetic lethal with cynT, rescued by high CO2. Can is reported to be a low abundance protein. 2/143 Can(YadF) peptide masses from MS analysis confirm this start codon and indicate that the initial Met is not removed (Maillet, 2007).

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BamHI EcoRI HindIII