GenePage for the intB' pseudogene of Escherichia coli K-12

Primary Gene Name: intB'
EcoGene Accession Number: EG12364
K-12 Gene Accession Number: ECK4264
MG1655 Gene Identifier: b4271
Gene Name Mnemonic: Integrase
Alternate Gene Symbols: int(P4); yjgC
Description: Pseudogene reconstruction, P4-like integrase
  # bp Upstream # bp Downstream
MW: 48114.32 ---------421 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4496675
Left Intergenic Region

Name: leuX_intB

Length: 185 bp gap

Orientation: Codirectional+

Left_end: 4496490

Right_end: 4496674

Centisome: 96.87

Genomic Address
Clockwise
Minute or Centisome (%) = 96.88
Right End: 4497940
Right Intergenic Region

Name: intB_insC11

Length: 331 bp gap

Orientation: Codirectional+

Left_end: 4497941

Right_end: 4498271

Centisome: 96.90

A stop codon at position 19 of intB' is replaced with an X to reconstruct. The intB' pseudogene is adjacent to the leuX tRNA gene and may be the remnant of P2-like phage integration at leuX and subsequent loss. The region from leuX to yjhQ gene was once designated as prophage KpLE2 (Hayashi, 2001). Although this region contains several IS elements, it has no other phage gene homologs and has many core genes, and thus is unlikely to represent a prophage region; therefore all references to the KpLE2 prophage have been removed. The region clockwise from leuX through intB' has been designated as the PR-X prophage remnant and described as P2-like and highly deleted, although no length or right end was indicated (Casjens, 2003). PR-X has not been annotated as a defective prophage because it appeqrs to be limited to one pseudogene, intB' and the leuX-intB' intergenic region (K.E.R.).

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BamHI EcoRI HindIII