GenePage for the ghoS gene of Escherichia coli K-12

Primary Gene Name: ghoS
EcoGene Accession Number: EG12468
K-12 Gene Accession Number: ECK4121
MG1655 Gene Identifier: b4128
Gene Name Mnemonic: Ghost cells
Alternate Gene Symbols: yjdK
Description: Antitoxin of GhoTS toxin-antitoxin pair; endonuclease for ghoT mRNA
  # bp Upstream # bp Downstream
MW: 11467.97 ---------98 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4352584
Left Intergenic Region

Name: yjdJ_ghoS

Length: 226 bp gap

Orientation: Codirectional+

Left_end: 4352358

Right_end: 4352583

Centisome: 93.77

Genomic Address
Clockwise
Minute or Centisome (%) = 93.77
Right End: 4352880
Right Intergenic Region

Name: ghoS_ghoT

Length: 27 bp gap

Orientation: Codirectional+

Left_end: 4352881

Right_end: 4352907

Centisome: 93.78

The GhoT(YjdO) toxin is a membrane-lytic protein that produces 60% non-viable ghost cells when overproduced and increases the formation of persister cells among the survivors; the GhoST toxin-antitoxin pair is proposed to define a new class of TA pairs, named type V, because the GhoS(YjdK) antitoxin is an RNase that cleaves the toxin mRNA; in addition to cleaving ghoT mRNA, 20 mRNAs encoding proteins involved in biosynthesis or transport of purines or pyrimidines are also apparently degraded by GhoS; the NMR structure of GhoS (PDB:2LLZ) resembles the Cas2 RNase of the CRISPR system (Wang, 2012; Van Melderen, 2012). Toxin endoribonuclease MqsR degrades ghoS antitoxin mRNA but not ghoT toxin mRNA, amplifying the GhoT toxin production as a stress response (Wang, 2013). MqsR induction increases GhoT production; GhoT is localized to poles and does not cause cell lysis during normal physiology but does impair membrane function, causing a reduction of cellular ATP and PMF energy sources, a lowered metabolism, and an increase in the number of persistent (dormant) cells formed in the presence of antibiotics (Cheng, 2013).

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BamHI EcoRI HindIII