GenePage for the ampH gene of Escherichia coli K-12

Primary Gene Name: ampH
EcoGene Accession Number: EG12867
K-12 Gene Accession Number: ECK0371
MG1655 Gene Identifier: b0376
Gene Name Mnemonic: Ampicillin
Alternate Gene Symbols: yaiH
Description: D-alanyl-D-alanine-carboxypeptidase/endopeptidase; peptidoglycan recycling or remodeling; penicillin-binding protein; very weak beta-lactamase
  # bp Upstream # bp Downstream
MW: 41849.03 ---------385 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 395130
Left Intergenic Region

Name: yaiV_ampH

Length: 0 bp

Orientation: Convergent

Left_end: Null

Right_end: Null

Centisome: 8.51

Genomic Address
Counterclockwise
Minute or Centisome (%) = 8.51
Right End: 396287
Right Intergenic Region

Name: ampH_sbmA

Length: 351 bp gap

Orientation: Divergent

Left_end: 396288

Right_end: 396638

Centisome: 8.54

The DD-endopeptidase activity of AmpH predominates in vitro and is 10- to 100-fold higher on natural substrates than the DD-carboxypeptidase activity; AmpH can act on the pentapeptide-rich macromolecular peptidoglycan in sacculi prepared from a multiple DD-carboxypeptidase mutant (González-Leiza, 2011). Although originally reported not to have beta-lactamase activity, a subsequent study demonstrated that AmpH has a very weak beta-lactamase activity using nitrocefin as the substrate; this activity is about 0.1% of the rate for AmpC, a known weak beta-lactamase (Henderson, 1997; González-Leiza, 2011). A cleavable 21 aa type I signal peptide for AmpH is strongly predicted by SignalP and cleavage at this site has been confirmed by mass loss observed using LC-MS, although since the mature N-terminus was not sequenced AmpH is not in the Verified Set; the 23 aa cleaved N-terminal sequence reported includes the N-terminal MG dipeptide added by the pET vector (González-Leiza, 2011). AmpH is firmly membrane-anchored and needs a strong detergent treatment to solubilize, however no transmembrane segments are predicted (González-Leiza, 2011).

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BamHI EcoRI HindIII