GenePage for the amiC gene of Escherichia coli K-12

Primary Gene Name: amiC
EcoGene Accession Number: EG13086
K-12 Gene Accession Number: ECK2813
MG1655 Gene Identifier: b2817
Gene Name Mnemonic: Amidase
Alternate Gene Symbols: ygdN
Description: N-acetylmuramyl-L-alanine amidase, periplasmic; recruited to the septal ring by FtsN during cell division; overexpression causes lysis; activated by NlpD
  # bp Upstream # bp Downstream
MW: 45634.45 ---------417 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2947757
Left Intergenic Region

Name: metV_amiC

Length: 73 bp gap

Orientation: Convergent

Left_end: 2947684

Right_end: 2947756

Centisome: 63.51

Genomic Address
Counterclockwise
Minute or Centisome (%) = 63.51
Right End: 2949010
Right Intergenic Region

Name: amiC_argA

Length: 231 bp gap

Orientation: Divergent

Left_end: 2949011

Right_end: 2949241

Centisome: 63.53

NlpD activates AmiC-mediated in vitro murein cleavage; nlpD and amiC mutants have the same phenotype, resulting from an AmiC defect (Uehara, 2010). AmiC is localized to the septal ring via its N-terminal septal targeting domain in constricting cells (Bernhardt, 2003). Divisome, non-essential. In amiABC triple mutants, thickened dark bands termed septal peptidoglycan (SP) rings appear at division sites, representing a cell division structure that accumulates when septal synthesis and hydrolysis by the AmiABC amidases are uncoupled (Priyadarshini, 2007). A mutant lacking five murein hydrolases (amiABCmltCD=MHD90) has a delayed lysis phenotype after beta-lactam treatment (Heidrich, 2002). AmiC is a verified Tat substrate (Bernhardt, 2003). AmiC has a predicted Tat-only (Class I) 31 aa signal peptide (Tullman-Ercek, 2007). Ize (2003) incorrectly report a predicted 33 aa signal peptide in the text, but correctly depict a predicted 31 aa signal peptide in Fig. 4A.

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BamHI EcoRI HindIII