GenePage for the arnA gene of Escherichia coli K-12

Primary Gene Name: arnA
EcoGene Accession Number: EG14091
K-12 Gene Accession Number: ECK2248
MG1655 Gene Identifier: b2255
Gene Name Mnemonic: ara4N (4-amino-4-deoxy-L-arabinose)
Alternate Gene Symbols: pbgP3(S.t.); pmrI(S.t.); SAF; yfbG
Description: UDP-glucuronate dehydrogenase and UDP-ara4N formyltransferase
  # bp Upstream # bp Downstream
Verified Start MW: 74288.89 ---------660 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2368039
Left Intergenic Region

Name: arnC_arnA

Length: 1 bp overlap

Orientation: Codirectional+

Left_end: 2368039

Right_end: 2368039

Centisome: 51.02

Genomic Address
Minute or Centisome (%) = 51.02
Right End: 2370021
Right Intergenic Region

Name: arnA_arnD

Length: 4 bp overlap

Orientation: Codirectional+

Left_end: 2370018

Right_end: 2370021

Centisome: 51.06

ArnA is a bifunctional enzyme involved in the modification of lipid A phosphates with aminoarabinose that is required for polymyxin B and cationic antimicrobial peptide resistance. ArnA catalyzes two steps in lipid A phosphate group modification by aminoarabinose: the C-terminal 345-residues encode the NAD+-dependent dehydrogenase domain that catalyzes the first step in UDP-4-amino-4-deoxy-L-arabinose synthesis and involves successive C4 oxidation and C6 decarboxylation steps; the N-terminal 304-residue domain encodes N-10 formyltetrahydrofolate-dependent UDP-aminoarabinose N-formyltransferase (UDP-formamidoarabinose synthase) that makes the substrate for ArnC: UDP-ara4FN = uridine 5'-diphospho-beta-(4-deoxy-4-formamido-L-arabinose). ArnA is identical to ssrA-associated factor SAF. basRS regulon. The arnBCADTEF operon promoter region binds FNR and arnBCADTEF expression is repressed by FNR (Federowicz, 2014).

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