GenePage for the cusF gene of Escherichia coli K-12

Primary Gene Name: cusF
EcoGene Accession Number: EG14234
K-12 Gene Accession Number: ECK0565
MG1655 Gene Identifier: b0573
Gene Name Mnemonic: Cu sensor
Alternate Gene Symbols: cusX; silF(S.t.); ylcC
Description: Silver- and cuprous copper-binding protein, periplasmic; efflux metallochaperone; confers copper and silver resistance; CusF binds both Cu(I) and Ag(I) with high affinity, but does not bind Cu(II)
  # bp Upstream # bp Downstream
Verified Start MW: 12251.16 ---------110 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 597131
Left Intergenic Region

Name: cusC_cusF

Length: 157 bp gap

Orientation: Codirectional+

Left_end: 596974

Right_end: 597130

Centisome: 12.86

Genomic Address
Clockwise
Minute or Centisome (%) = 12.86
Right End: 597463
Right Intergenic Region

Name: cusF_cusB

Length: 15 bp gap

Orientation: Codirectional+

Left_end: 597464

Right_end: 597478

Centisome: 12.87

Soluble periplasmic CusF binds Cu(I) specifically but weakly, probably explaining why it was not detected using Cu(I)-MIRAGE (Kittleson, 2006; Sevcenco, 2009; Sevcenco, 2012). cusF is in the cusCFBA Cu/Ag efflux operon regulated by Cu and Ag. The first 22 aa are predicted to be a type I signal peptide. However, N-terminal sequencing revealed cleavage after residue 21; the CusF clone construct had eight additional amino acids added at the N-terminus; the cleavage specificity may have been altered by the additional amino acids or the prediction is incorrect (Fanake, 2003).

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BamHI EcoRI HindIII