GenePage for the insX' pseudogene of Escherichia coli K-12

Primary Gene Name: insX'
EcoGene Accession Number: EG14418
K-12 Gene Accession Number: ECK4473
MG1655 Gene Identifier: b4505
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: ykgN
Description: Pseudogene, IS3 family transposase, ISX' in CP4-6 prophage; N-terminal fragment
  # bp Upstream # bp Downstream
MW: 9517.78 ---------83 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 280114
Left Intergenic Region

Name: insI2_insX

Length: 9 bp gap

Orientation: Convergent

Left_end: 280105

Right_end: 280113

Centisome: 6.03

Genomic Address
Counterclockwise
Minute or Centisome (%) = 6.03
Right End: 280362
Right Intergenic Region

Name: insX_yagB

Length: 64 bp gap

Orientation: Codirectional-

Left_end: 280363

Right_end: 280426

Centisome: 6.04

InsX' is the N-terminus of an IS3 family transposase A encoded in the partial IS3-related ISX' element in the putative defective prophage CP4-6. InsX' matches 81 of the first 83 aa of Shigella flexneri ISEhe3-like 92 aa orfA transposase (UniProtKB: Q0T4M3). The ISEhe3 prototype in Pantoea agglomerans (GenBank: AF327445) encodes a 94 aa orfA transposase (UniProtKB: Q0T4M3) that is 90% identical (75/83 aa) to InsX'. The Q0T4M3 start codon corresponds to a GTG 2 codons upstream of the insX' ATG start codon. However this GTG does not have as good an RBS as the annotated insX' ATG start codon.

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII