GenePage for the iroK gene of Escherichia coli K-12

Primary Gene Name: iroK
EcoGene Accession Number: EG14501
K-12 Gene Accession Number: ECK4518
MG1655 Gene Identifier: b4706
Gene Name Mnemonic: Unknown
Alternate Gene Symbols: None
Description: 3-hydroxypropionic acid resistance peptide
  # bp Upstream # bp Downstream
MW: 2329.79 ---------21 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2668006
Left Intergenic Region

Name: hcaT_iroK

Length: 159 bp gap

Orientation: Codirectional-

Left_end: 2667847

Right_end: 2668005

Centisome: 57.48

Genomic Address
Counterclockwise
Minute or Centisome (%) = 57.48
Right End: 2668071
Right Intergenic Region

Name: iroK_hcaR

Length: 66 bp overlap

Orientation: Codirectional-

Left_end: 2668006

Right_end: 2668071

Centisome: 57.48

The last 21 amino acids of the hcaR gene has been proposed to be a separate IroK protein whose gene is nested in hcaR that when expressed increases resistance to 3-hydroxypropionic acid independent of HcaT-mediated transport; an apparent RBS in front of iroK is inferred to initiate translation from a clone and when the starting ATG is mutated to TTG the resistance phenotype goes away, however expression of a peptide from the chromosomal iroK locus was not demonstrated (Warnecke, 2011).

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BamHI EcoRI HindIII