GenePage for the istR gene of Escherichia coli K-12

Primary Gene Name: istR
EcoGene Accession Number: EG31167
K-12 Gene Accession Number: ECK4425
MG1655 Gene Identifier: b4616
Gene Name Mnemonic: inhibitor of SOS-induced toxicity
Alternate Gene Symbols: psr19; psrA19
Description: sRNAs IstR-1 and IstR-2, tisB regulators
  # bp Upstream # bp Downstream
MW: 45130.87 ---------140 nt Pre-Run BlastN
Left End: 3853118
Left Intergenic Region

Name: ivbL_istR

Length: 129 bp gap

Orientation: Codirectional-

Left_end: 3852989

Right_end: 3853117

Centisome: 83.01

Genomic Address
Counterclockwise
Minute or Centisome (%) = 83.01
Right End: 3853257
Right Intergenic Region

Name: istR_tisB

Length: 295 bp gap

Orientation: Divergent

Left_end: 3853258

Right_end: 3853552

Centisome: 83.01

IstR-1 (75 bp) is initiated from an internal promoter; lstR-2 is lexA-regulated, possibly mitigating the lstR-1 antisense inhibition of residual TisB translation during the SOS response (Vogel, 2004). The antisense sRNA IstR binds to and blocks an upstream ribosome loading standby site required for translation initiation of TisB; ribosomes probably slide to the highly structured downstream TIR after loading; this results in RNase III cleavage of the active, processed +42 tisB mRNA to form an inactive +106 tisB mRNA (Darfeuille, 2007).

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BamHI EcoRI HindIII