GenePage for the agrB gene of Escherichia coli K-12

Primary Gene Name: agrB
EcoGene Accession Number: EG31190
K-12 Gene Accession Number: ECK4570
MG1655 Gene Identifier: b4713
Gene Name Mnemonic: ArsR-Gor Region
Alternate Gene Symbols: None
Description: Antisense sRNA antitoxin for the DinQ toxin
  # bp Upstream # bp Downstream
MW: 27125.26 ---------84 nt Pre-Run BlastN
Left End: 3648294
Left Intergenic Region

Name: agrA_agrB

Length: 147 bp gap

Orientation: Codirectional+

Left_end: 3648147

Right_end: 3648293

Centisome: 78.60

Genomic Address
Clockwise
Minute or Centisome (%) = 78.60
Right End: 3648377
Right Intergenic Region

Name: agrB_arsR

Length: 150 bp gap

Orientation: Codirectional+

Left_end: 3648378

Right_end: 3648527

Centisome: 78.60

DinQ is a 27 amino acid UV-inducible toxin that depolarizes the cell membrane, decreases ATP concentration, and affects nucleoid structure after UV exposure; DinQ and the agrB sRNA antitoxin constitute a novel type I-like toxin-antitoxin module that affects the cell response to UV-induced DNA damage; three lines of evidence demonstrate that the agrA sRNA antitoxin can neutralize the DinQ toxin: (1) an agrA deletion mutant is UV-sensitive, grows slowly, and is recessive, while the dinQ agrAB deletion strain is not UV-sensitive and grows normally (agrA single mutants have no phenotype, indicating that the agrA sRNA is inactive), (2) a clone with both agrA and dinQ does not display the UV-sensitivity caused by introducing a dinQ clone alone into wildtype cells, and (3) a dinQ clone cannot be introduced into an agrB mutant strain (Weel-Sneve, 2013). The TERM55 intergenic repeat with transcription termination activity corresponds to the agrA sRNA terminator (Blaisdell, 1993; Rudd, 1999).

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BamHI EcoRI HindIII