GenePage for the insO' pseudogene of Escherichia coli K-12

Primary Gene Name: insO'
EcoGene Accession Number: EG40121
K-12 Gene Accession Number: ECK4476
MG1655 Gene Identifier: b4623, b4562, b4285, b4283
Gene Name Mnemonic: Insertion Sequence
Alternate Gene Symbols: insN2; ORFAB; yjhW
Description: Pseudogene reconstruction, fused IS911 transposase AB
  # bp Upstream # bp Downstream
MW: 39917.63 ---------389 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4507197
Left Intergenic Region

Name: yjhE_insO

Length: 87 bp gap

Orientation: Codirectional+

Left_end: 4507110

Right_end: 4507196

Centisome: 97.10

Genomic Address join

The following join statement uses GenBank syntax to describe how the reconstructed insO pseudogene protein sequence was translated from the DNA:

join(4507197..4507451,4509009..4509479,4509479..4509793)


Clockwise
Minute or Centisome (%) = 97.10
Right End: 4509793
Right Intergenic Region

Name: insO_insI4

Length: 14 bp gap

Orientation: Convergent

Left_end: 4507452

Right_end: 4507465

Centisome: 97.11

The individual ORFA and ORFB proteins are also observed, but the fused ORFAB protein is the active transposase. insO is interrupted by the insertion of IS30D at codon 86, also the site of translation frameshifting in the intact ORFAB. 43 aminos acids are deleted at the insertion site, represented by Xs. A frameshift mutation caused by a one base pair deletion in codon 285 was hypothetically corrected by translating codon 285 as X instead of the H that was probably there prior to the one base pair deletion event; frame is preserved in the gene reconstruction by repeating the nucleotide prior to the deleted nucleotide. The annotated start site was verified for a wildtype IS911. The last seven residues of ORFB are polymorphic; the C-terminal residues of this reconstructed ORFAB from K-12 matches the C-terminus of ORFB from UniProtKB M9F4Q3. Fused versions of ORFAB include UniProtKB Q6Q6S9 and Q8GJ19, but with different C-terminal residues than K-12.

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BamHI EcoRI HindIII